Regulatory

Part:BBa_J31020:Design

Designed by: Erin Zwack   Group: iGEM06_Davidson   (2008-05-09)


produces taRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 150
    Illegal XbaI site found at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 150
    Illegal XbaI site found at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 150
    Illegal XbaI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the sequence had to be modified to contain the SpeI/XbaI scar in the region that complements the crRNA


Source

sequence was adapted from a paper by Isaacs et al. 2004 on riboregulators

References